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Becker & Hickl spc-150 photon counting card
Spc 150 Photon Counting Card, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc-150/tcspc+module/pmc11725929-330-22-28
Average 90 stars, based on 1 article reviews
spc-150 photon counting card - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Fluorescence:

Article Title: Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM
Article Snippet: Fluorescence was then detected by the time-resolved detector connected to TCSPC electronics (SPC-150 and DCC-100, Becker & Hickl GmbH), which was synchronized with the laser signal to facilitate precise calibration of time delays.

Article Title: Hyperautofluorescent material inside areas of macular atrophy may reveal non-lipofuscin fluorophores in late stage AMD.
Article Snippet: Fluorescence photons were detected by time- correlated single photon counting (SPC- 150; Becker & Hickl GmbH, Berlin, Germany) in a short- wavelength (SSC: 498–560 nm) and a long- wavelength (LSC: 560–720 nm) spectral channel.

Article Title: Spin effect in the ferromagnetic organic photovoltaics cells
Article Snippet: In organic photovoltaic devices, the separation and transport of photogenerated charges play crucial roles for power conversion efficiency.. Magnetic doping in organic solar cells can effectively enhance the power conversion efficiency by introducing a static magnetic field.. In this study, we observed that in pure organic magnetic solar cells, the spin-polarization-induced spin scattering effect can also efficiently modulate the photocurrent in solar cells.

Article Title: Phase separation and molecular ordering of the prion-like domain of the thermosensory protein EARLY FLOWERING 3
Article Snippet: Fluorescence was collected after a pinhole of 100 μ m diameter size (P100D, Thorlabs) by an avalanche photodetector (SPCM-AQR-15, PerkinElmer) connected to an SPC-150 (Becker & Hickl) TCSPC card.

Article Title: Impact of cigarette smoking on fluorescence lifetime of ocular fundus
Article Snippet: Two highly sensitive hybrid detectors (HPM-100-40; Becker & Hickl, Berlin, Germany) register the detected emission photons in a short spectral channel (SSC: 498–560 nm) and a long spectral channel (LSC: 560–720 nm) that are connected to the TCSPC module (SPC-150, Becker & Hickl) for photon counting.

Article Title: Multimodal non-invasive probing of stress-induced carotenogenesis in the cells of microalga Bracteacoccus aggregatus.
Article Snippet: Microalgae are the richest source of natural carotenoids—accessory photosynthetic pigments used as natural antioxidants, safe colorants, and nutraceuticals.. Microalga Bracteacoccus aggregatus IPPAS C-2045 responds to stresses, including high light, with carotenogenesis—gross accumulation of secondary carotenoids (the carotenoids structurally and energetically uncoupled from photosynthesis).. Precise mechanisms of cytoplasmic transport and subcellular distribution of the secondary carotenoids under stress are still unknown.

Article Title: A modular chemigenetic calcium indicator for multiplexed in vivo functional imaging
Article Snippet: The FLIM board used was SPC-150 (Becker & Hickl), and time domain single-photon counting was performed in 256 time channels.

Article Title: Detecting Early Degradation of Wood Ultrastructure with Nonlinear Optical Imaging and Fluorescence Lifetime Analysis
Article Snippet: Two-photon excited autofluorescence was revealed using a photomultiplier tube (PMH-100, Becker & Hickl GmbH, Berlin, Germany) routed to a time-correlated single-photon counting (TCSPC, SPC-150, Becker & Hickl) acquisition card that records the fluorescence intensity decay for each pixel.

Droplet Countercurrent Chromatography:

Article Title: Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM
Article Snippet: Fluorescence was then detected by the time-resolved detector connected to TCSPC electronics (SPC-150 and DCC-100, Becker & Hickl GmbH), which was synchronized with the laser signal to facilitate precise calibration of time delays.

Article Title: Hyperautofluorescent material inside areas of macular atrophy may reveal non-lipofuscin fluorophores in late stage AMD.
Article Snippet: Fluorescence photons were detected by time- correlated single photon counting (SPC- 150; Becker & Hickl GmbH, Berlin, Germany) in a short- wavelength (SSC: 498–560 nm) and a long- wavelength (LSC: 560–720 nm) spectral channel.

Article Title: Spin effect in the ferromagnetic organic photovoltaics cells
Article Snippet: In organic photovoltaic devices, the separation and transport of photogenerated charges play crucial roles for power conversion efficiency.. Magnetic doping in organic solar cells can effectively enhance the power conversion efficiency by introducing a static magnetic field.. In this study, we observed that in pure organic magnetic solar cells, the spin-polarization-induced spin scattering effect can also efficiently modulate the photocurrent in solar cells.

Article Title: Phase separation and molecular ordering of the prion-like domain of the thermosensory protein EARLY FLOWERING 3
Article Snippet: Fluorescence was collected after a pinhole of 100 μ m diameter size (P100D, Thorlabs) by an avalanche photodetector (SPCM-AQR-15, PerkinElmer) connected to an SPC-150 (Becker & Hickl) TCSPC card.

Article Title: Impact of cigarette smoking on fluorescence lifetime of ocular fundus
Article Snippet: Two highly sensitive hybrid detectors (HPM-100-40; Becker & Hickl, Berlin, Germany) register the detected emission photons in a short spectral channel (SSC: 498–560 nm) and a long spectral channel (LSC: 560–720 nm) that are connected to the TCSPC module (SPC-150, Becker & Hickl) for photon counting.

Article Title: Multimodal non-invasive probing of stress-induced carotenogenesis in the cells of microalga Bracteacoccus aggregatus.
Article Snippet: Microalgae are the richest source of natural carotenoids—accessory photosynthetic pigments used as natural antioxidants, safe colorants, and nutraceuticals.. Microalga Bracteacoccus aggregatus IPPAS C-2045 responds to stresses, including high light, with carotenogenesis—gross accumulation of secondary carotenoids (the carotenoids structurally and energetically uncoupled from photosynthesis).. Precise mechanisms of cytoplasmic transport and subcellular distribution of the secondary carotenoids under stress are still unknown.

Article Title: A modular chemigenetic calcium indicator for multiplexed in vivo functional imaging
Article Snippet: The FLIM board used was SPC-150 (Becker & Hickl), and time domain single-photon counting was performed in 256 time channels.

Article Title: Detecting Early Degradation of Wood Ultrastructure with Nonlinear Optical Imaging and Fluorescence Lifetime Analysis
Article Snippet: Two-photon excited autofluorescence was revealed using a photomultiplier tube (PMH-100, Becker & Hickl GmbH, Berlin, Germany) routed to a time-correlated single-photon counting (TCSPC, SPC-150, Becker & Hickl) acquisition card that records the fluorescence intensity decay for each pixel.



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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Image Search Results


Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Journal: Biomedicines

Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

doi: 10.3390/biomedicines12112550

Figure Lengend Snippet: Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

Techniques: Membrane, Viscosity, Control

Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Journal: Biomedicines

Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

doi: 10.3390/biomedicines12112550

Figure Lengend Snippet: Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

Techniques: Membrane, Control